Akademik Veri Yönetim Sistemi
Tezin Türü: Doktora
Tezin Yürütüldüğü Kurum: Dokuz Eylül Üniversitesi, Sağlık Bilimleri Enstitüsü, Türkiye
Tezin Onay Tarihi: 2021
Tezin Dili: Türkçe
Öğrenci: ASUMAN FEDA BAYRAK
Danışman: Zekiye Sultan Altun
Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
Özet:
This study aims to determine and
evaluate whether the administration of Darbepoetin (DPO) has a preventive
effect against ototoxic effects due to cisplatin on Wistar rats. DPO’s
potential protective effect against ototoxicity will be examined through
nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and target genes.
In the study; 28 wistar albino rats were used to form, control, cisplatin
(16mg/kg IP ), DPO (25 ug/kg, IP), DPO administered before cisplatin groups, Apoptotic
cell death was determined by TUNEL analysis by in situ hybridization in ear
tissue; caspase 3, 8, 9 levels immunohistochemical methods, Nrf-2 and Nrf-2
associated with NrQ2 antioxidant and iNOS, nNOS, protein expressions will be
studied by tissue immunohistochemical methods. Each group will be evaluated in
comparison.
With cisplatin application,
hearing loss was detected in all frequencies showing ototoxic effects compared
to the control group. In the examinations performed at the level of
immunohistochemical tissue, structural changes in the inner ear, necrosis and
necroptosis in the brain and nerve tissues were detected in the ciplatin group.
Effects of darbopeotin on nitric oxide system while cisplatin prevented the
inner ear and brain damage, both apoptotic protein expressions and nrf-2,
associated with oxidative stress, on NQO1 was also detected.
In this study, ototoxicity,
which consists of cisplatin has been shown at functional level. Darbopoetin has
been shown to protect against inner ear and brain damage due to cisplatin
reducing apoptosis, increasing anti-oxidant protein levels, preventing
oxidative stress.