TO COMPARE THE DIFFERENT SCAFFOLDS ACCORDING TO POROSITY'S PROPERTIES


ÇEÇEN B., Kozaci L. D., Yuksel M., Pasinli A., Oral O., HAVITÇIOĞLU H.

14th National Biomedical Engineering Meeting, İzmir, Turkey, 20 - 22 May 2009, pp.296-297 identifier identifier

  • Publication Type: Conference Paper / Full Text
  • Volume:
  • Doi Number: 10.1109/biyomut.2009.5130324
  • City: İzmir
  • Country: Turkey
  • Page Numbers: pp.296-297
  • Keywords: Scaffolds, Osteoblast, PLLA, HA
  • Dokuz Eylül University Affiliated: Yes

Abstract

The main purpose of this study is to investigate the increase in osteoblast cells cultured on three different porosity scaffolds in vivo environment. Hence, Collagen + Poly-l-lactic acid (PLLA), Collagen +HA (30 C degrees), Collagen + HA (37 C degrees) were studied. During the operation, 20 ml bone marrow was taken for any of patient in sterile conditions. Mesenchymal stem cells were differentiated to osteoblast cells. After the ALP and morphological evaluations were assessed, osteoblast cells were planted on 3 different scaffolds and SEM observations were evaluated. At twenty-fifth day, konfleunt osteoblast cells were observed. Osteoblast cells were watched on Collagen + HA (30 C degrees) scaffold appropriately. According to our knowledge, in various bone defects, cell cultures on suitable scaffolds are contribute to clinical studies.