Research Information System
25th IUBMB, 46th FEBS and 15th PABMB The Biochemistry Global Summit, Lisbon, Portugal, 9 - 14 July 2022, pp.141
This study aimed to investigate the possible renoprotective effects
of taurine via p38 MAPK and TGF-b/smad2/3 signaling pathways in a diabetic nephropathy model in rats. 29 Wistar albino
rats were divided into 4 groups: control, taurine (1% with drinking water), diabetes (45 mg/kg Streptozotosin), diabetes+taurine.
After 12 weeks, kidney and serum were collected for biochemical
and histological studies. The histological analysis showed that
there were significant changes in tubule dilatation and loss of
tubule brush border in the kidney of the diabetes group. These
changes were significantly decreased with taurine. However, there
were no significant changes in serum creatinine and blood urine
nitrogen levels among groups. Further, taurine significantly
reduced the protein expression of NADPH oxidase 4, known as
a major enzymatic source for oxidative stress in the kidney. Also,
there was a significant decrease in reactive oxygen species and
malondialdehyde levels by taurine whereas the decreased superoxide dismutase activity level in the diabetes group was significantly increased with taurine. On the other hand, taurine resulted
in significant decreases in both mRNA and protein expression of
fibronectin, which is a major extracellular matrix protein related
to the fibrosis process, These findings were parallel to Masson
Trichrome staining. The matrix metalloproteinases (MMP)-2 and
MMP-9 mRNA expression levels and their activities increased
significantly in diabetes compared to the control, and these
increases were significantly reached to higher levels with taurine.
Also, the decreased mRNA expression of extracellular matrix
metalloproteinase inducer (EMMPRIN) increased with taurine in
the diabetes+taurine group. Moreover, it is found that taurine
suppressed the p38 MAPK and TGF-b/smad2/3 signaling pathways. All these findings indicate that taurine may be an effective
practical strategy to prevent renal diabetic injury