Preparation and characterization of radiolabeled magnetic nanoparticles as an imaging agent

İÇHEDEF Ç., TEKSÖZ S., ÜNAK P., MEDİNE E. İ., ERTAY T., BEKİŞ R.

JOURNAL OF NANOPARTICLE RESEARCH, cilt.14, sa.8, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 8
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s11051-012-1077-0
  • Dergi Adı: JOURNAL OF NANOPARTICLE RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Magnetic nanoparticles, Guanine, Technetium tricarbonyl core, Imaging, IRON-OXIDE NANOPARTICLES, IN-VITRO, PRECURSOR, COMPLEX, RE-188, PROBE
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Magnetic nanoparticles were prepared by a reduction-precipitation method and coated with an amino silane coupling agent. Guanine (Gua) was conjugated to the magnetic nanoparticles (MNPs) using glutaraldehyde as a cross-linker. Common techniques (Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and vibrating electron microscopy) were used to assess the properties of the particles. Structural investigations showed that amino silane-coated MNPs had a particle size of about 40-60 nm in diameter with a spherical morphology. The guanine-conjugated MNPs were radiolabeled with Tc-99m(I)-tricarbonyl core (Tc-99m(CO)(3)-MNP-Gua) with a labeling yield of 72 +/- 4 %. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of Tc-99m(CO)(3)-MNP-Gua was 98 +/- 2 % in the final solution. The biologic distribution of guanine MNPs was assessed in New Zealand rabbits using a gamma camera. In the in vivo experiment, a high level of radioactivity was observed in the lungs and liver soon after intravenous administration of Tc-99m(CO)(3)-MNP-Gua.