Pinealectomy alters IFN-γ and IL-10 levels in primary thymocyte culture of rats


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ŞAHİN Z., SANDAL S., Yilmaz B., Bulmus O., Ozdemir G., Kutlu S., ...Daha Fazla

Cellular and Molecular Biology, cilt.64, sa.14, ss.25-30, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 14
  • Basım Tarihi: 2018
  • Doi Numarası: 10.14715/cmb/2018.64.14.5
  • Dergi Adı: Cellular and Molecular Biology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.25-30
  • Anahtar Kelimeler: Cytokine, Melatonin, Pineal gland, Rat, T helper, Thymocyte
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Melatonin, produced mainly by the pineal gland, has an immunomodulatory role. However, the effects of the pineal gland and/or melatonin on thymus cytokine levels such as interferon-gamma (IFN-γ), interleukin (IL)-4, and IL-10 are not well known. Twenty-one male Wistar rats (220-250 gr) were randomly divided into three groups (n=7): intact control, sham, and pinealectomy. Primary thymocyte cultures were prepared from each group and dispensed into well plates as Control, DMSO (or vehicle), Sham-pinealectomy, Pinealectomy, Pinealectomy+10μM melatonin, and Pinealectomy+100μM melatonin. IFN-γ, IL-4, and IL-10 concentrations were measured in the thymocytes (as nonstimulated and Concanavalin A-stimulated) after 24 h. IFN-γ levels significantly increased and IL-10 levels significantly decreased in both media prepared from pinealectomized rats. There was no significant difference between the groups in terms of IL-4. In the pinealectomy+10μM melatonin group, IFN-γ and IL-10 levels did not differ from the pinealectomy group. However, the dose of 100μM melatonin caused a decrease in levels of IFN-γ in both thymocyte media and an increase in the concentration of IL-10 in Concanavalin A-stimulated thymocytes. In conclusion, pineal gland and/or melatonin affect IFN-γ and IL-10 levels in the thymus gland.