Akademik Veri Yönetim Sistemi
Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.18, sa.6, ss.951-955, 2012 (SCI-Expanded)
The objective of this experiment was to determine the ruminal binding characteristics of modified S. cerevisiae extract and hydrated sodium calcium aluminosilicate (HSCAS) containing mycotoxin adsorbent (MA) against various aflatoxins in an in vitro study. A certified aflatoxin mixture (B1, G1, B2, G2) in a liquid form was mixed with ruminal in vitro medium providing the final concentrations of 6 ng/ml aflatoxin B1 (AFB1), 6 ng/ml aflatoxin G1 (AFG1), 1.5 ng/ml aflatoxin B2 (AFB2), and 1.5 ng/ml aflatoxin G2 (AFG2). Treatments were: 1) aflatoxin mixture + distilled water (Control); 2) aflatoxin mixture + rumen fluid (AR); and 3) aflatoxin mixture + MA (6 mg) + rumen fluid (AMAR). After various incubation time points (0, 3, 6, 12, 24 h) at 39°C, aflatoxin concentrations in ruminal medium were detected with HPLC. Although AFB1 concentration at 0 h was 6 ng/ml, it was reduced to 2.50 and 1.68 ng/ml in Control, 0.86 and 0.50 ng/ml in AR, and 0.34 and 0.20 ng/ml in AMAR treatments at 3 and 12 h, respectively (P<0.001). In addition, AFB1 concentration in AMAR treatment was in a steady-state condition after 3 h of incubation compared to Control and AR treatments where AFB1 concentration became stabilized after 12 h of incubation. A similar type of binding pattern was also observed for AMAR treatment in ruminal incubation of AFB2. In addition, the concentrations of both AFG1 and AFG2 were in a steady-state condition for AR (0.67 and 0.48 ng/ml) and AMAR (0.46 and 0.38 ng/ml) treatments after 12 h of ruminal incubation. The binding capability of the MA on AFG1 and AFG2 was always in favor of AMAR treatment at all time points. There was no treatment effect on ruminal in vitro gas production across all treatments, averaging 53.5 ml at 24 h. Results indicate that the MA can help binding the studied aflatoxins and reducing their concentrations in the rumen before they enter into the bloodstream.