Akademik Veri Yönetim Sistemi
IUBMB-FEBS-PABMB 2022 Congress, Lisbon, Portekiz, 9 - 14 Temmuz 2022, ss.60, (Özet Bildiri)
In our study, we aimed to evaluate the relationship between pos-sible changes in glycolysis metabolism and the energy-dependentAMP kinase and Akt signaling pathway during neuronal differ-entiation of human wharton jelly-derived mesenchymal stem cells(WJ-MSC). During the differentiation process, time-dependentchanges in cellular lactate, pyruvate, AMPK enzyme and AcetylCoA levels were measured by colorimetric photometric and fluo-rometric method in the presence and absence of Akt, which inac-tivates the AMPK enzyme. On the 7th day of neuronaldifferentiation induction, AMPK enzyme levels were significantlydecreased (p<0.05), and the AMPK levels increased after Aktinhibitor application. In WJ-MSCs, it was shown that cellularlactate levels decreased depending on the induction time(p<0.05). On the 7th day of neuronal differentiation, it wasdetermined that pyruvate levels decreased significantly comparedto cells without induction group, and acetyl CoA levels wereincreased. While this decrease observed in pyruvate levels wasprevented by Akt inhibition, it was observed that acetyl CoAlevels increased even more. The significant decrease in AMPKlevels on the 7th day when neural progenitor cells are formed inthe differentiation process supports the hypothesis that thisenzyme, which suppresses lipid synthesis, may play a key role inthe formation of mature neurons. However, the change in acetylCoA and pyruvate levels observed with inhibition of Akt sug-gested that the Akt-AMPK signaling pathway may play a domi-nant role in acetyl CoA metabolism. On the other hand, in thepresence of Akt, the capacity of WJ-MSCs to transform intomature neurons may be promoted, along with a decrease in theAMPK enzyme level and an increase in the utilization of pyru-vate. Further studies will guide the use of lactate-acetyl CoA/pyruvate levels as a biomarker in neuronal differentiation stepsin WJ-MSCs.