Development of the magnetic beads for dye ligand affinity chromatography and application to magnetically stabilized fluidized bed system

DEMİR M. N., Akdogan F., Kalburcu T., Akgol S., DENİZLİ A.

PROCESS BIOCHEMISTRY, vol.45, no.4, pp.556-562, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 4
  • Publication Date: 2010
  • Doi Number: 10.1016/j.procbio.2009.12.001
  • Journal Name: PROCESS BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.556-562
  • Keywords: Dye-affinity beads, Magnetic polymers, Protein purification, beta-Casein, Magnetic beads, BETA-CASEIN, ANTIBODY PURIFICATION, HUMAN SERUM, ADSORBENT, ADSORPTION, FIELD, REMOVAL, ALBUMIN
  • Dokuz Eylül University Affiliated: Yes

Abstract

Magnetic poly(2-hydroxyethylmethacrylate) [mPHEMA] beads were prepared by suspension polymerization of HEMA in the presence of Fe(3)O(4) nano-powder. Cibacron Blue F3GA was covalently immobilized to the mPHEMA beads via nucleophilic substitution reaction between chloride of its triazine ring and hydroxyl groups of HEMA under alkaline conditions. The mPHEMA/Cibacron Blue F3GA beads (100-140 mu m in diameter) carrying 68.3 mu mol Cibacron Blue F3GA per gram polymer were used for beta-casein adsorption studies. Adsorption studies were performed under different conditions in a batch system (i.e., pH beta-casein initial concentration, temperature, and ionic strength) and then in a magnetically stabilized fluidized bed (MSFB) system. The swelling ratio of the mPHEMA was 62.1%. The maximum adsorption capacity for batch system was 20.2% lower as compared to the value obtained in MSFB. The mPHEMA/Cibacron Blue F3GA beads could be repeatedly applied for beta-casein adsorption without significant losses in the adsorption capacity. (C) 2009 Elsevier Ltd. All rights reserved.