Akademik Veri Yönetim Sistemi
TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, cilt.29, sa.3, ss.637-642, 2009 (SCI-Expanded, Scopus)
Objective: Pseudomonas aeruginosa may show adaptive pathogenicity according to microenvironment conditions. Biofilm formation is an important virulence characteristic. In this study, effect of aminoglycoside treatment on biofilm formation and its relation with quorum sensing (QS) in P. aeruginosa were questioned. Material and Methods: Three different cell lines (VERO; HEp-2, RD) were infected with P. aeruginosa [multiplicity of infection (m.o.i): 50:1] and were treated with three different aminoglycosides in supra-MIC (minimum inhibitory concentration), MIC, 50% MIC concentrations. Following antibiotic treatments, QS responses were determined by micro AHL method and biofilm formation was evaluated spectrophotometrically. Results: Three aminoglycosides stimulated biofilm formation in P. aeruginosa in each cell line. In VERO and HEp-2 cell lines, QS systems of ATCC 27853, C1 and C2 strains were active under all antibiotic concentrations. In RD cell line, las and rhl systems of P. aeruginosa ATCC 27853 were active; however, las and rhl systems of C1 and C2 strains were not active under gentamicin effect. las system of C1 and rhl system of C2 were active under amikacin effect, and all QS systems of C1 and C2 worked under netilmicin effect. Conclusion: P. aeruginosa may "behave" in different patterns in different microenvironments. For three P. aeruginosa strains, biofilm formation was detected in all cell lines. In epithelial cell lines, QS response was different from the response in muscle cell line. This comparative modeling may be useful for the development of new antimicrobials against P. aeruginosa.