Bio-hydrogen production by photo-fermentation of dark fermentation effluent with intermittent feeding and effluent removal

ÖZMIHÇI S., Kargi F.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.35, no.13, pp.6674-6680, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 13
  • Publication Date: 2010
  • Doi Number: 10.1016/j.ijhydene.2010.04.090
  • Journal Name: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.6674-6680
  • Keywords: Bio-hydrogen, Continuous photo-fermentation, Dark fermentation effluent, Hydraulic residence time, Rhodobacter sphaeroides, HYDROLYZED WHEAT-STARCH, BIOHYDROGEN PRODUCTION, GROUND WHEAT, PHOTOHYDROGEN PRODUCTION, CLOSTRIDIUM-BUTYRICUM, LIGHT-INTENSITY, H-2 PRODUCTION, GAS-PRODUCTION, CULTURE, KINETICS
  • Dokuz Eylül University Affiliated: Yes

Abstract

Pure culture of Rhodobacter sphaeroides (NRRL- B1727) was used for continuous photofermentation of volatile fatty acids (VFA) present in the dark fermentation effluent of ground wheat starch. The feed contained 1950 +/- 50 mg L-1 total VFA with some nutrient supplementation. Hydraulic residence time (HRT) was varied between 24 and 120 hours. The highest steady-state daily hydrogen production (55 ml d(-1)) and hydrogen yield (185 ml H-2 g(-1) VFA) were obtained at HRT = 72 hours (3 days). Biomass concentration increased with increasing HRT. Volumetric and specific hydrogen formation rates were also maximum at HRT = 72 h. High extent of TVFA fermentation at HRT = 72 h resulted in high hydrogen gas production. (C) 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.