DETERMINATION OF TRACE METAL IONS USING PORPHYRINS AS CHELATING AGENTS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY-DENSITOMETRY

SEYHAN BOZKURT S., Cavdar I. K., Kurtbay H. M., MERDİVAN M.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.33, sa.6, ss.748-760, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 6
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1080/10826071003684018
  • Dergi Adı: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.748-760
  • Anahtar Kelimeler: high performance thin layer chromatography, metal, porphyrin, solid phase extraction, LIQUID-CHROMATOGRAPHY, RP-HPLC, MICROWAVE DIGESTION, MASS-SPECTROMETRY, SILICA-GEL, EXTRACTION, SEPARATION, COMPLEXES, PALLADIUM, CELLULOSE
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

A method was developed for separation and quantitative determination of mercury, copper, cobalt, and zinc as porphyrin complexes using newly synthesized tetra-(3-bromo-4-hydroxyphenyl) porphyrin (TBHPP), tetra-(4-phenoxyphenyl) porphyrin (TPPP) and tetra-p-chloromethylphenyl porphyrin (CMPP) in aqueous medium by HPTLC densitometry. The method involves enrichment of metal ions as metal complexes with octadecyl cartridges and elution using tetrahydrofuran. The analysis was performed on HPTLC silica gel plates with acetone-chloroform (2:8, v/v) as the mobile phase for TBHPP and TPPP quantification and dichloromethane-chloroform-hexane (1:1:3, v/v) for CMPP quantification. Quality parameters were confirmed and detection limits were ranged from 0.16 to 0.92ngL-1. Detection was performed densitometrically in absorption-reflection mode around 420nm. The method was found to be simple, precise and accurate for the determination of these metal ions in water and food sample.