Effect of Iron Deficiency and Supplemented Conditions on the Antioxidant System, Membrane Lipid Peroxidation and Some Metal Levels in Spirulina maxima


ÖZTÜRK ÜREK R., Tarhan L.

JOURNAL OF PURE AND APPLIED MICROBIOLOGY, cilt.5, sa.2, ss.593-602, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 2
  • Basım Tarihi: 2011
  • Dergi Adı: JOURNAL OF PURE AND APPLIED MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.593-602
  • Anahtar Kelimeler: Spirulina maxima, Phycocyanin, Superoxide dismutase, Ascorbate-dependent peroxidase, Lipid peroxidation, Iron, SUPEROXIDE-DISMUTASE, SYNECHOCOCCUS SP, PHOTOSYNTHESIS, CYANOBACTERIUM, TOXICITY
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Effects of iron deficiency (10 mu M) and excess (50-100 mu M) conditions, causing oxidative stress, on enzymatic and non-enzymatic antioxidants, also lipid peroxidation (LPO) were investigated in blue-green cyanobacterium, Spirulina maxima depending on time. Increasing iron concentration in the medium resulted in increases in intracellular iron levels. Uptakes of iron and magnesium indicated positive correlation, whereas Mn levels in deficiency condition showed a similar trend with control and increased in excess conditions. While catalase activity wasn't observed in all conditions, the highest superoxide dismutase and ascorbate-dependent peroxidase activities were determined in 50 mu M on 13(th) day. However, chlorophyll contents in deficiency condition were similar to control up to 13th day, they were over control in excess conditions. Then, they tended to increase in all conditions except control. Phycocyanin contents in deficiency condition up to 7 day were slightly lower than controls' though significantly higher than it in excess conditions. Then, phycocyanin levels for control were determined over all conditions, except 17(th) day, for excess conditions. Ascorbate and total carotenoid contents showed similar, but converse trends, depending on iron concentration except control. Control's LPO levels indicated insignificant change, yet they in other conditions were higher than control's and showed decreasing variations during time.