Use of ionic liquid based chitosan as sorbent for preconcentration of fluoroquinolones in milk, egg, fish, bovine, and chicken meat samples by solid phase extraction prior to HPLC determination

SEYHAN BOZKURT S., Erdogan D., Antep M., DEMİR M. N., MERDİVAN M.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, vol.39, no.1, pp.21-29, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.1080/10826076.2015.1116010
  • Journal Name: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.21-29
  • Keywords: Antibiotic, chitosan, fluoroquinolone, HPLC, ionic liquid, solid phase extraction, TANDEM MASS-SPECTROMETRY, CLOUD POINT EXTRACTION, WATER SAMPLES, WASTE-WATER, SELECTIVE EXTRACTION, MICROEXTRACTION METHOD, QUINOLONE RESIDUES, CARBON NANOTUBES, AQUEOUS SAMPLES, HONEY SAMPLES
  • Dokuz Eylül University Affiliated: Yes

Abstract

The possibility of using ionic liquid based chitosan sorbent for the separation and preconcentration of fluoroquinolone antibiotics (marbofloxacin, enoxacin, ofloxacin, ciprofloxacin, and enrofloxacin) has been studied. For this reason, different ionic liquids were prepared and coated on the chitosan sorbent. The conditions of the preconcentration of fluoroquinolones on a microcolumn have been optimized and the extraction efficiencies of the prepared sorbents have been compared. The compounds were eluted with 5 mL of 20% NH3 (v/v, MeOH) solution and determined by HPLC with diode array and fluorescence detector. The limits of detection were found as 4.23 mu g L-1 for marbofloxacin, and 1.09 mu g L-1 for enoxacin; 3.23 x 10(-3) mu g L-1 for ofloxacin; 8.39 x 10(-3) mu g L-1 for ciprofloxacin; and 19.50 x 10(-3) mu g L-1 for enrofloxacin. The developed method was applied for the analysis of fluoroquinolone in milk, egg, fish, bovine, and chicken samples and the recoveries were obtained in the range 70-100%.