Research Information System
OPTICS2023 2nd International Conference and Expo on Lasers, Optics & Photonics, Barcelona, Spain, 9 - 10 October 2023, (Unpublished)
The Lateral Flow Immunoassay (LFIA) is an important paper-based platform in point-of-care (POC), easy-to-use, cost-effective, and rapid diagnostic test in a variety of disciplines to detect the target analytes in samples consisting of fresh blood, plasma, or serum. Nonetheless, its practical applicability is impeded by the inherent inability of standard LFIAs to deliver measurable information that is quantifiable and many immunochromatography methods have a binary presence/absence detection, which restricts insights into distinct disease phases. This study attempts to overcome this limitation by introducing an optical imaging method that studies the progression of specific diseases within a patient in an efficient and affordable way. CdTe quantum dots (QDs) are embedded in LFIA conjugated pads (glass fiber) since they are important II-VI group semiconductor material with a narrow bulk band gap corresponding to 1.5 eV, a high excitation Bohr radius, and size-dependent properties which make them ideal for use in various biomedical applications. Observed samples were classified into 2 categories: (1) aqueous forms of 1:1, 1:2, 1:10, and 1:100 dilutions of CdTe QD with a stock concentration of 3.5 mg/ml diluted with borate buffer at pH 7.5, and (2) dry forms embedded in conjugate pads with a volume of 4 µl at the identical dilutions. The setup built for aqueous forms includes a 405-nm LED source, sample holder, 490-nm LP dichroic-mirror, and spectrometer. A comparative analysis reveals that glass fiber medium caused a 21.27-fold reduction in the PL Intensities of CdTe quantum dots. Using a 405 nm laser source instead of LED to observe dry forms improved the results by 967.8%. Rising QD concentrations cause aggregation-induced emission, which may aid in determining the disease level in a patient body. The half-life of strips was measured as 13 days. Due to pad size limits, the introduced arrangement retrieves previously unachievable data in XRD and spectrofluorometer. This study has the potential to improve diagnostic platforms by overcoming the limitations of traditional procedures and offers promising potential for next-generation rapid diagnosis.