Electrochemical investigation of hydroxyapatite-lanthanum strontium cobalt ferrite composites (HA-LSCF) for SARS-CoV-2 aptasensors

Hartati Y. W., Devi M. J., Irkham I., Zulqaidah S., Noviyanti A. R., Rochani S., ...More

RSC ADVANCES, vol.13, no.29, pp.20209-20216, 2023 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 29
  • Publication Date: 2023
  • Doi Number: 10.1039/d3ra01531a
  • Journal Name: RSC ADVANCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, Chemical Abstracts Core, Compendex, Metadex, Directory of Open Access Journals
  • Page Numbers: pp.20209-20216
  • Dokuz Eylül University Affiliated: No

Abstract

The hydroxyapatite-lanthanum strontium cobalt ferrite (HA-LSCF) composite showed a good response on a screen-printed carbon electrode (SPCE) electrochemical aptasensor to detect SARS-CoV-2. SPCE/HA-LSCF with a thiolated aptamer has a strong affinity for the SARS-CoV-2 spike RBD protein. This occurs due to the binding of -SH to the HA-positive region. In the presence of LSCF, which is conductive, an increase in electron transfer from the redox system [Fe(CN)(6)](3-/4-) occurs. The interaction of the aptamer with the RBD protein can be observed based on the decrease in the electron transfer process. As a result, the developed biosensor is highly sensitive to the SARS-CoV-2 spike RBD protein with a linear range of 0.125 to 2.0 ng mL(-1), a detection limit of 0.012 ng mL(-1), and a quantification limit of 0.040 ng mL(-1). The analytical application of the aptasensor demonstrates its feasibility in the analysis of saliva or swab samples.