Development of a novel IMAC sorbent for the identification of melamine in dairy products by HPLC

Ozturk S., Demir N.

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, cilt.100, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 100
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.jfca.2021.103931
  • Dergi Adı: JOURNAL OF FOOD COMPOSITION AND ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Melamine, Immobilized metal-chelate affinity chromatog-raphy, Solid phase extraction, Dairy product, SOLID-PHASE EXTRACTION, PERFORMANCE LIQUID-CHROMATOGRAPHY, CYANURIC ACID, IMPRINTED POLYMER, MILK POWDER, ADSORPTION, REMOVAL, ELECTROCHEMILUMINESCENCE, CYROMAZINE, MECHANISM
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

To determine melamine (MEL) in dairy products, a novel immobilized metal affinity chromatography (IMAC) sorbent using chitosan (CTS) and 2-hydroxyethyl methacrylate (HEMA) as monomers, ethylene glycol dimethacrylate (EGDMA) as cross-linker and Cu2+ ions as chelated metal ion was synthesized by radical polymerization and characterized by Fourier transfer infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and swelling test. Adsorption parameters (pH, contact time, initial concentration, etc.) were studied to evaluate the adsorption process. The results showed that the CTS-p(HEMA)-Cu2+ IMAC sorbent displayed a rapid dynamic adsorption with a maximum adsorption capacity of 275.49 mu g g-1 toward melamine. The CTS-p(HEMA)-Cu2+ IMAC sorbent was also applied to determine and enrich melamine in dairy products (milk and infant formula). An enrichment ratio of 5-fold, detection limit of 0.005 and 0.015 mu g mL-1, and quantification limit of 0.0150 and 0.1261 mu g mL-1 were achieved for MEL in milk and infant formula, respectively.