FRESENIUS ENVIRONMENTAL BULLETIN, cilt.28, ss.1281-1286, 2019 (SCI-Expanded)
An extraction and analytical method for the determination of melamine in powdered milk using ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (UPLC/MS/MS) has been developed. In the method, the melamine was extracted using a perchloric acid (HCLO4), clean up sample supernatant using Oasis (R) MCX cartridge (3 cc 60 mg, P/N 186000254), and the final extract was analyzed in a single injection by UPLC/MS/MS. Limit of quantification (LOQ) value of melamine was found to be lower than the maximum residue limits (MRL). Matrix-matched calibration curves were applied and correlation coefficients (R-2) was greater than 0.99. The recovery values were found to be between 70 and 100% with relative standard deviations less than 20%. Based on these results, the proposed method is suitable for the routine determination of the concentration of melamine in powdered milk.