Varied Approach of Using MSCs for Bovine Embryo In Vitro Culture

Opiela J., Bulbul B., Romanek J.

ANIMAL BIOTECHNOLOGY, cilt.31, sa.1, ss.1-8, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/10495398.2018.1448224
  • Dergi Adı: ANIMAL BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-8
  • Anahtar Kelimeler: Bovine, embryo in vitro culture, medium conditioning, MSC, MESENCHYMAL STEM-CELLS, BIOACTIVE MATERIALS, CONDITIONED MEDIUM, MATURATION, CALCIUM, QUALITY, ORIGIN
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

The objective of the present study was to evaluate the effect of porcine mesenchymal stem cells (MSCs) secreted factors on bovine in vitro embryo development using MSCs in different culture systems: SOF medium, SOF medium conditioned by MSCs in monolayer, and in reverse drop and by embryo culture in coculture with MSCs. Statistically highly significant differences were noted between the number of blastocysts derived cultures in all tested culture systems. The in vitro culture in SOF turned out to be the most optimal. Statistically highly significant differences were observed in the number of blastocyst obtained between SOF and SOF in coculture with MSCs (P < 0.0001), and between SOF and SOF conditioned (monolayer and drop) (P < 0.00001). The trials to produce blastocysts in SOF conditioned by MSCs in reverse drops and monolayer failed. The blastocysts were obtained and analyzed by TUNEL only in two out of four experimental groups: SOF and SOF in coculture with MSCs. There were no significant differences between any of analyzed blastocysts' groups neither in the total number of nuclei nor in the apoptotic features. Neither medium conditioning by MSCs in monolayer and in reverse drop nor embryo culture in coculture with MSC turned out to be effective.