Enzymatic phenolics removal by tyrosinase-modified micromotors

Öndeş B., SUNNA Ç., Ki̇li̇mci̇ U., Uygun D. A., Uygun M.

Process Biochemistry, vol.147, pp.101-108, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 147
  • Publication Date: 2024
  • Doi Number: 10.1016/j.procbio.2024.08.012
  • Journal Name: Process Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.101-108
  • Keywords: Micromotor, Phenolic Removal, Tyrosinase
  • Dokuz Eylül University Affiliated: Yes

Abstract

In this study, self-propelled Pt/PPy-COOH micromotors were produced by using the sequential electrodeposition of Pt and PPy segments. Fabricated micromotors were modified with tyrosinase enzyme and was used to investigate the phenolic compound removal capacity. The micromotors was characterized by SEM and EDX analyses, and it was found that the shape of the motors was tubular and 10 µm long. The optimum pH values of free and immobilized enzymes were found to be 7.5, while the optimum temperature values were 45 °C. In the thermal stability study, it was determined that the immobilized enzyme showed 75 % activity after 5 h, while the activity of the free enzyme was around 21 %. Also, immobilized tyrosinase showed 55 % activity after 10 repeated uses. Finally, the phenol, p-cresol and o-phenylenediamine removal efficiencies of tyrosinase-based micromotors were found to be 54.54 %, 46.96 % and 53.87 % in 5 min, respectively. As a result, it can be concluded that it is possible to use tyrosinase-based micromotors as an alternative to traditional methods for phenol removal.