Objective: The relationship between sexual functions and infertility remains controversial and there is a limited number of studies reporting the effects of infertility on sexual functions in women with polycystic ovary syndrome (PCOS). Aims: The present study aims to investigate the effects of infertility on sexual functions and depression levels in women with PCOS. Methods: A total of 150 participants who were either fertile patients with PCOS, infertile patients with PCOS, or fertile women without PCOS (control) (n = 50) were included for the study. Sociodemographic data were recorded and the Female Sexual Function Index (FSFI) and Beck Depression Inventory (BDI) were used for evaluation of subjects. Results: Body mass index (BMI) was found to be significantly higher in the PCOS plus infertility group (27.9 ± 2.9, P = 0.01) than the other groups. Total BDI score was higher and total FSFI score was significantly lower in the PCOS plus infertility group than in the other groups. The PCOS plus infertility group showed significantly lower FSFI scores than the PCOS group in terms of desire, lubrication, orgasm, satisfaction, and pain. A significant negative correlation was observed between BMI and BDI scores in the PCOS plus infertility group (r:-0.384, P = 0.04). Conclusion: Our study results show lower sexual functions in PCOS women with infertility and that infertility negatively affects sexual functions with obesity associated depression.