Cytoprotective effect of trimetazidine on 75 min warm renal ischemia-reperfusion injury in rats

Ozden A., Aybek Z., Saydam N., Calli N., Saydam O., Duzcan E., ...More

EUROPEAN SURGICAL RESEARCH, vol.30, no.4, pp.227-234, 1998 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 4
  • Publication Date: 1998
  • Doi Number: 10.1159/000008581
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.227-234
  • Keywords: renal ischemia, ischemia-reperfusion injury, trimetazidine, malondialdehyde, glutathione, glutathione peroxidase, OXYGEN FREE-RADICALS, SUPEROXIDE-DISMUTASE, LIPID-PEROXIDATION, DAMAGE, ACCUMULATION, GLUTATHIONE, PREVENTION, NEUTROPHIL, KIDNEYS, RABBITS
  • Dokuz Eylül University Affiliated: No


In this experimental study, we evaluated the effect of trimetazidine (TMZ) on renal ischemia-reperfusion (IR) injury in Sprague-Dawley rats. Renal IR was achieved by a 75-min clamping of the left renal pedicle and subsequent 24 h reperfusion, after right nephrectomy was performed. The rats were randomly divided into three groups: group 1 (sham operated: no IR injury), group 2 (ischemic control: saline treatment), and group 3 (3 mg/kg TMZ before ischemia). After 24 h of reperfusion, blood samples and renal tissue samples were taken to measure the levels of creatinine, tissue malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) activity. Histopathological changes were evaluated. In addition, the 7-day survival rates in each group were evaluated. We found significant increases in the levels of creatinine and tissue MDA, severe acute tubular necrosis, and a significant decrease in the activity of the GSH-Px in group 2. There were significant decreases in the levels of creatinine and tissue MDA, mild acute tubular necrosis, and a significant increase in activity of the GSH-Px in group 3 when compared with the control group (p <0.05). Statistically significant differences (p <0.05) in survival were noted between the ischemic control and sham-operated and TMZ groups. We have concluded that TMZ is able to protect the kidney from warm IR injury.