Cardioprotective effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in rats

Bicakci N., Karaboga I., Dokmeci A. H., Guzel S., Erboga Z. F.

BIOTECHNIC & HISTOCHEMISTRY, vol.94, no.6, pp.442-448, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 94 Issue: 6
  • Publication Date: 2019
  • Doi Number: 10.1080/10520295.2019.1586999
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.442-448
  • Keywords: Apoptosis, caffeic acid phenethyl ester, cardiac contusion, contusion, rats, TNF-alpha, troponin, I, TNF-ALPHA, PULMONARY CONTUSION, MYOCARDIAL-ISCHEMIA, INDUCED COLITIS, LUNG CONTUSION, KAPPA-B, PATHWAY, MODEL, ANTIOXIDANT, COMBINATION
  • Dokuz Eylül University Affiliated: No


We investigated the effects of caffeic acid phenethyl ester (CAPE) on cardiac damage after blunt chest injury. Forty male adult Wistar albino rats were divided into four groups; control, cardiac contusion, cardiac contusion + CAPE, and CAPE. CAPE, 10 mmol/kg, was administered intraperitoneally for 7 days following cardiac contusion. Heart tissue and blood were obtained at the end of the experimental period. Cardiac histopathology was determined using hematoxylin & eosin (H & E) staining. Expression of tumor necrosis factor-alpha (TNF-alpha) in cardiomyocytes was determined using immunohistochemistry. Cardiac apoptosis was determined using the TUNEL method. Serum creatine kinase (CK), creatine kinase-muscle/brain (CK-MB) and lactate dehydrogenase (LDH) levels were determined using spectrophotometric methods. The serum cardiac troponin I (C-TI) level was measured using the ELISA method. Myofibril loss was detected in the cardiomyocytes of the cardiac contusion group. Increased apoptosis and TNF-alpha expression were observed in the cardiac contusion group compared to the control group. Increased CK, CK-MB, LDH and C-TI levels were found in the cardiac contusion group. We found that CAPE administration improved myocardial function. Compared to the cardiac contusion group, CK, CK-MB, LDH and C-TI levels decreased significantly in the cardiac contusion + CAPE group. Administration of CAPE significantly inhibited apoptosis and cardiac TNF-alpha expression. Our findings demonstrate the therapeutic effects of CAPE for cardiac contusion damage after blunt chest trauma.