In this study, a glucose sensor modified with activated carbon supported gold-nickel (AuNi@AC) metal nanoparticles was prepared for the early diagnosis of diabetes. Electrochemical tests were carried out by determining the optimum working conditions of the prepared glucose sensor. The characterization analyses of the designed glucose sensor were performed by Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), X-Ray Photoelectron Spectroscopy (XPS) and Raman Spectroscopy. It was determined that the average particle size of the nanoparticles in the AuNi alloy structure was 2.03 +/- 0.37 nm. The determined detection limit of the AuNi@AC nanosensor was calculated as 0.41 mu M as a result of the high linear range provided up to 1.7 mM. In addition, the sensitivity of AuNi@AC nanosensor to glucose, which has a high sensitivity value of 1955 mu A mM-1 cm-2, was determined.