A novel amperometric biosensor based on artichoke (Cynara scolymus L.) tissue homogenate immobilized in gelatin for hydrogen peroxide detection

Ozturk G., Ertas F., AKYILMAZ E., Dinckaya E., Tural H.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.32, sa.4, ss.637-645, 2004 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 4
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1081/labb-200039660
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.637-645
  • Anahtar Kelimeler: hydrogen peroxide, biosensor, artichoke (Cynara scolymus L.), tissue homogenate based electrode, amperometry, ELECTRODES
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

A biosensor for specific determination of hydrogen peroxide was developed by using homogenized artichoke (Cynara scolymus L.) tissue in combination with a dissolved oxygen probe and applied in determination of hydrogen peroxide in milk samples. Artichoke tissue, which has catalase activity, was immobilized with gelatine by means of glutaraldehyde and fixed on a pretreated teflon membrane. The electrode response was maximum when 0.05 M phosphate buffer was used at pH 7.0 and at 30degreesC. Upon addition of hydrogen peroxide, the electrode gives a linear response in a concentration range of 5.0-50x10(-5) M with a response time of 3 min. The method was also applied to the determination of hydrogen peroxide in milk samples.