A novel amperometric biosensor based on artichoke (Cynara scolymus L.) tissue homogenate immobilized in gelatin for hydrogen peroxide detection


Ozturk G., Ertas F., AKYILMAZ E., Dinckaya E., Tural H.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.32, no.4, pp.637-645, 2004 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 4
  • Publication Date: 2004
  • Doi Number: 10.1081/labb-200039660
  • Journal Name: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.637-645
  • Keywords: hydrogen peroxide, biosensor, artichoke (Cynara scolymus L.), tissue homogenate based electrode, amperometry, ELECTRODES
  • Dokuz Eylül University Affiliated: Yes

Abstract

A biosensor for specific determination of hydrogen peroxide was developed by using homogenized artichoke (Cynara scolymus L.) tissue in combination with a dissolved oxygen probe and applied in determination of hydrogen peroxide in milk samples. Artichoke tissue, which has catalase activity, was immobilized with gelatine by means of glutaraldehyde and fixed on a pretreated teflon membrane. The electrode response was maximum when 0.05 M phosphate buffer was used at pH 7.0 and at 30degreesC. Upon addition of hydrogen peroxide, the electrode gives a linear response in a concentration range of 5.0-50x10(-5) M with a response time of 3 min. The method was also applied to the determination of hydrogen peroxide in milk samples.