Molecularly Imprinted Polypyrrole for DNA Determination

Ratautaite V., TOPKAYA ÇETİN S. N., Mikoliunaite L., Ozsoz M., Oztekin Y., Ramanaviciene A., ...Daha Fazla

ELECTROANALYSIS, cilt.25, sa.5, ss.1169-1177, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 5
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1002/elan.201300063
  • Dergi Adı: ELECTROANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1169-1177
  • Anahtar Kelimeler: Molecularly imprinted polymers (MIPs), Polypyrrole, DNA, Electrochemical DNA biosensor, DNA-imprinted polymer
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

In this study graphite electrodes modified by a thin DNA-imprinted polypyrrole layer, which was able to bind specific target-DNA, are reported. For this aim, electrochemical synthesis of polypyrrole was performed on a pencil graphite electrode by cyclic voltammetry (CV) or by potential pulse sequences (PPS). The modified electrode surface was used for electrochemical determination of target-DNA by differential pulse voltammetry. According to our best knowledge this is a first report on the application of DNA-imprinted polymer for the determination of target-DNA. The results showed that the molecularly imprinted polypyrrole (MIPPy) layer that formed on the carbon electrode surface was sensitive for target-DNA, while the nonimprinted polypyrrole layer was not sensitive to the same target-DNA. Comparison of electrodes modified using PPS and CV techniques is presented.