Molecularly Imprinted Polypyrrole for DNA Determination
ELECTROANALYSIS, vol.25, no.5, pp.1169-1177, 2013 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 25 Issue: 5
- Publication Date: 2013
- Doi Number: 10.1002/elan.201300063
- Journal Name: ELECTROANALYSIS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.1169-1177
- Keywords: Molecularly imprinted polymers (MIPs), Polypyrrole, DNA, Electrochemical DNA biosensor, DNA-imprinted polymer
- Dokuz Eylül University Affiliated: No
Abstract
In this study graphite electrodes modified by a thin DNA-imprinted polypyrrole layer, which was able to bind specific target-DNA, are reported. For this aim, electrochemical synthesis of polypyrrole was performed on a pencil graphite electrode by cyclic voltammetry (CV) or by potential pulse sequences (PPS). The modified electrode surface was used for electrochemical determination of target-DNA by differential pulse voltammetry. According to our best knowledge this is a first report on the application of DNA-imprinted polymer for the determination of target-DNA. The results showed that the molecularly imprinted polypyrrole (MIPPy) layer that formed on the carbon electrode surface was sensitive for target-DNA, while the nonimprinted polypyrrole layer was not sensitive to the same target-DNA. Comparison of electrodes modified using PPS and CV techniques is presented.