Efficacy of an adenosine A, receptor agonist compared with atropine and pralidoxime in a rat model of organophosphate poisoning


Kalkan Ş., Ergür B. U., Arıcı M. A., Kaplan Y. C., Kınay A. Ö., Tunçok Y.

HUMAN & EXPERIMENTAL TOXICOLOGY, cilt.24, sa.7, ss.369-375, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 7
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1191/0960327105ht540oa
  • Dergi Adı: HUMAN & EXPERIMENTAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.369-375
  • Anahtar Kelimeler: atropine, organophosphate, PAM, phenylisopropyl adenosine (PIA), NECROSIS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

The objective of this study was to evaluate the effects of an adenosine A, agonist, phenylisopropyl adenosine (PIA), on metamidophos poisoning compared to specific antidotes. Rats were poisoned with metamidophos (30 mg/kg, oral) and observed for 24 hours. One group received sodium chloride (1 mL/kg) and four experimental groups received atropine (5 mg/kg), pralidoxime (PAM, 20 mg/kg), atropine/PAM (5/20 mg/kg) or PIA (1 mg/kg) intraperitoneally. Atropine reduced salivation and prevented respiratory distress when compared to sodium chloride-treated rats. Treatment with PAM did not cause any suppression of cholinergic signs. Atropine and PAM combination prevented salivation, convulsion and respiratory distress. PIA delayed initial time of the salivation, convulsion and time to death. However, PIA was found ineffective against the metamidophos-induced cholinergic symptoms and mortality. All treatments, except PIA, lead to survival of these animals. Acetylcholinesterase (AChE) activity was not normalized by PIA or PAM. PIA prevented metamidophos-induced diaphragmatic muscle necrosis as much as PAM. In conclusion, a single dose of PIA was unable to protect the rats from metamidophos toxicity. Further studies are needed involving a combination of PAM and/or atropine with repeated doses of PIA to clarify the efficacy of adenosine agonists in OP poisoning.