Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status

Bayrak S., Altun Z. S., İnce D., Aktaş S., Olgun N.

UHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISI, cilt.29, sa.2, ss.70-78, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 2
  • Basım Tarihi: 2019
  • Doi Numarası: 10.4999/uhod.193154
  • Dergi Adı: UHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.70-78
  • Anahtar Kelimeler: Betulinic acid, N-MYC, Metastases genes, Neuroblastoma, Cisplatin, ACETYL L-CARNITINE, INDUCED APOPTOSIS, RETINOIC ACID, CANCER, ACTIVATION, PROLIFERATION, EXPRESSION, INVASION, BCL-2, P53
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Betulinic acid-(BA) has apoptotic effects on neuroblastoma. This study explored the effects of cisplatin-(CP), BA and combination of BA and CP (BA-CP) on cell proliferation, apoptotic cell death and expressions of metastasis related genes in neuroblastoma. In this study, N-MYC positive and negative neuroblastoma cell lines, KELLY and SHSY5Y, were treated with BA, CP and BA-CP combinations. BA-CP combinations showed synergistic activity on cell growth inhibition and apoptotic cell death in both neuroblastoma cell lines. In SHSY5Y cells BA, CP or BA-CP treatments induced downregulation most of the metastatic genes' expressions. In KELLY cells, while CP treatment decreased some metastatic gene expressions, BA or BA-CP treatment reduced only DENR and RB1. BA and BA-CP showed prominent effect on metastasis related gene expressions in predominantly KELLY cells. The results of this study suggest that BA may be a promising anti-cancer agent for neuroblastoma treatment, whereas CP is still the most suitable chemotherapeutic drug for aggressive neuroblastoma.