Reference values for T and B lymphocyte subpopulations in Turkish children and adults
Turkish journal of medical sciences, cilt.51, sa.4, ss.1814-1824, 2021 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 51 Sayı: 4
- Basım Tarihi: 2021
- Doi Numarası: 10.3906/sag-2010-176
- Dergi Adı: Turkish journal of medical sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.1814-1824
- Anahtar Kelimeler: Immunophenotyping, lymphocyte subsets, reference values, lymphocyte percentage, absolute count
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
Background/aim: Established reference values are critical for the interpretation of immunologic assessments. In particular, the proportion and absolute counts of T-and B-cell subpopulations are subject to change with age and ethnicity. We aimed to establish age-specific reference values for lymphocyte subsets using updated immunophenotyping panels. Materials and methods: We studied a total of 297 healthy Turkish subjects aged 0 to 50 years, stratified into major age brackets in a cluster factor of 10 per age-group. The predetermined age intervals contained randomly allocated participants enrolled over a period of 6 months, who were homogenously distributed by sex. We analyzed a complete blood count test and simultaneously with detailed immunophenotyping enumerated the percent and absolute cell counts of lymphocyte subsets. Results: The percentage and absolute counts of lymphocyte subsets show a marked surge across the age-span. T helper, T cytotoxic, and the natural killer cell numbers were increasing from birth until 6 months, followed by a gradual decrease thereafter. B cell numbers were rising until 2 years, followed by a gradual decrease for the upcoming years, accompanied by a steady expansion of unclass-switched-and class-switched-B cells. Discussion: We provide updated extensive reference intervals for lymphocyte subpopulations in Turkish people.