Evaluation of methylation status. in glutation S-transferase P1(GSTP1) gene promoter in human breast cancer and it relation to tumor grade and stage

Shargh S. A., Mohaddes S. M., Sakizli M., Gharesouran J., Karami F.

RESEARCH JOURNAL OF CHEMISTRY AND ENVIRONMENT, cilt.15, sa.2, ss.492-497, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 2
  • Basım Tarihi: 2011
  • Dergi Adı: RESEARCH JOURNAL OF CHEMISTRY AND ENVIRONMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.492-497
  • Anahtar Kelimeler: GSTP1, promoter, methylation, pattern, breast cancer, DNA METHYLATION, HAPLOTYPES, MODULATION, CELLS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Glutation S-transferase P1 (GSTP1) gene methylation in promoter CpG islands has been described as a specific biomarker for many type of cancer including breast cancer as a tumor suppressor gene. In present study we found the GSTP1 gene promoter to be methylated in breast cancer tissues. To study the effect of sequence variation on hypermethylated GSTP1 promoter in cancer tissues and non methylated one in normal tissues, we analyzed the cytosine methylation status as epigenetic changes in 50 tumors from patient's with breast cancer and 50 normal breast tissues taken from the same breast tumor's adjacent region. In order to study the promoter methylation status for GSTP1 gene in breast cancer, 40 CpG sites [nucleotide(nt) 197,190,187,185,183,182,176,162,155,152,148,145,141, 132,127, 124,112,109,101,99,81,77,74,71,54,53,48, 47;43,42,40,38,23,22,15,14,13,11,8,4] were screened.