A new technique to avoid losing the strains of Echinococcus multilocularis during passaging


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Inceboz T., Korkmaz M., Uner A.

SAUDI MEDICAL JOURNAL, vol.26, no.12, pp.1911-1913, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 12
  • Publication Date: 2005
  • Journal Name: SAUDI MEDICAL JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1911-1913
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: To examine the survival of Echinococcus multilocularis (E. multilocularis) and the formation of protoscoleces, under refrigerated conditions over 43-day observation period 
Methods: We conducted this study in Ege University, School of Medicine, from May-December, 2004. In this experimental study, we aimed to define a technique to keep the strains during these passages. We found that preserved viability and virulence of E. multilocularis stored in RPMI 1640 plus 10% fetal calf serum at +40C.
Results: We accomplished the infection of the Meriones unguiculatus after in-vivo passaging from that
flask, which we kept at +40C, and this proves the viability of protoscoleces and membranous structures perfectly at the 43rd day. 
Conclusion: We also found that E. multilocularis metacestodes and the formation of protoscoleces keep their viability up to 60 days and virulence up to 43 days under this condition. We recommend storing an extra flask during each passage as a precaution against losing strains.