Lung-On-A-Chip Technologies for Disease Modelingand Drug Development

Konar D., Devarasetty M., YILDIZ D. V., Atala A., Murphy S.

Biomedical Engineering and Computational Biology, vol.7, no.S1, pp.17-27, 2016 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: S1
  • Publication Date: 2016
  • Doi Number: 10.4137/becb.s34252
  • Journal Name: Biomedical Engineering and Computational Biology
  • Journal Indexes: Emerging Sources Citation Index (ESCI)
  • Page Numbers: pp.17-27
  • Keywords: 3D culture, lung organoid, microfluidics, microscopy, EMBRYONIC STEM-CELLS, IN-VITRO, EPITHELIAL-CELLS, CULTURE MODELS, TISSUE, MATRIX, BARRIER, FATE, DIFFERENTIATION, GENERATION
  • Dokuz Eylül University Affiliated: Yes

Abstract

Animal and two-dimensional cell culture models have had a profound impact on not only lung research but also medical research at large, despite inherent flaws and differences when compared with in vivo and clinical observations. Three-dimensional (3D) tissue models are a natural progression and extension of existing techniques that seek to plug the gaps and mitigate the drawbacks of two-dimensional and animal technologies. In this review, we describe the transition of historic models to contemporary 3D cell and organoid models, the varieties of current 3D cell and tissue culture modalities, the common methods for imaging these models, and finally, the applications of these models and imaging techniques to lung research.