Microbiological Standardization in Small Laboratory Animals and Recommendations for the Monitoring

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JOURNAL OF CLINICAL AND ANALYTICAL MEDICINE, vol.6, no.5, pp.673-677, 2015 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 5
  • Publication Date: 2015
  • Doi Number: 10.4328/jcam.2195
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.673-677
  • Keywords: Microbiological Standardization, Mice, Rat, FELASA Recommendation, Health Monitoring Program, MICE, RATS
  • Dokuz Eylül University Affiliated: Yes


Microbiological standardization in laboratory animal breeding is based on the classification according to the microorganisms that the animals host and consequently their upbringing environment, as well as the certification of their microbiological status and the protection of their properties. Although there are many different classifications for microbiological standardization of laboratory animals, they can be basically classified as; gnotobiotic animals, animals bred with a complete barrier system (Germ free, GF, with Colonization-Resistant Flora; CRF), animals bred with a partial barrier system (Specified Pathogen Free, SPF), and animals bred by conventional methods in units without barriers (Conventional; CV). Monitoring of microbiological standardization is carried out in two ways. One is controlling barrier systems (process control) and the other is controlling laboratory animals (product control). In controlling barrier systems samples are taken routinely from ambient air, surfaces, base plate materials of animals, foods and waters, and microbiological tests are carried out. FELASA guidelines are frequently used in monitoring laboratory animals. These guidelines where the monitoring frequency, sample size, micro-organisms to be tested, vary according to the microbiological quality of the animals, and test methods and are frequently updated by FELASA and shared in their web pages. In our country, in general, laboratory animals used for experimental studies present no microbiological standardization, and follow-up protocols are not implemented. Therefore, construction of facilities for the production of microbiologically standard animals and establishment of backup laboratories testing microbiological quality should be established.