Research Information System
TURKISH JOURNAL OF BIOCHEMISTRY, vol.35, no.3, pp.248-255, 2010 (SCI-Expanded)
Objectives: The main aim of the present study was to evaluate the cortisol levels in gingival crevicular fluid by high performance liquid chromatography method. Secondary aim was to compare the cortisol levels in several periodontal diseases. Methods: Fifteen chronic periodontitis and 15 aggressive periodontitis patients were recruited. A total of 90 gingival crevicular fluid samples were collected from 3 teeth that had shallow, moderate and deep probing pocket depth of each patient. Gingival crevicular fluid samples were analyzed by high performance liquid chromatography method. Results: Gingival crevicular fluid samples of shallow pockets had lower volume (0.05µL>) than deep pockets (1-0.5 µL). Gingival crevicular fluid cortisol levels of shallow pockets were under the limit of detection. On the other hand, some of the gingival crevicular fluid cortisol levels of moderate and deep pocket samples were under the limit of detection. In this respect, it is hard to analyze and compare the cortisol levels of gingival crevicular fluid samples. Conclusions: Within the limitations of this study, it may be suggested that high performance liquid chromatography method is not an acceptable method to evaluate gingival crevicular fluid samples and to compare the cortisol levels in several periodontal diseases.