Investigation of the presence of equine herpesvirus-1 (EHV-1) in tissue samples of the aborted foals using histopathological, immunohistochemical, and Real- Time PCR techniques

Deniz S., Gulcubuk A., SÖNMEZ K., Altan E., Danyer l. A., Gurel A., ...Daha Fazla

JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.74, sa.4, 2023 (SCI-Expanded, Scopus)

Özet

Equine herpesvirus-1 (EHV-1) causes some manifestations in horses including; pneumonia, encephalitis, and abortion of live or dead feti in the last trimester of pregnancy. The aim of this study is to investigate the incidence of EHV-1 infection in the tissue samples collected from the aborted stillborn and live-born foals and also the foals which died soon after birth, as well as to compare and correlate the conventional histopathologic diagnosis with IHC and Real-Time PCR. For this purpose, tissue samples (lung, liver, spleen, kidney, placenta, and brain) of 38 foals from Istanbul, Kocaeli, Bursa, and Eskisehir provinces of Turkey between August 2009 and March 2011 were examined using IHC and Real-Time PCR. The obtained results showed 52.6 % positive Real-Time PCR rests (20/38 of examined samples) and 39.4 % positive immunohistopathological (IHC) tests (15/38 of examined samples), which indicated a correlation between these two diagnostic methods. In histopathological evaluation, the inclusion body was found in at least one of the tissue sections belonging to the lung, spleen, and mostly liver of 20 foals (52.6%). Tissue samples of the foals found to be positive by IHC and PCR revealed histopathologic findings compatible with the characteristics of the disease.