Wash-free, peptide-based fluorogenic probes for microbial imaging

Baibek A., ÜÇÜNCÜ M., Blackburn E. A., Bradley M., Lilienkampf A.

PEPTIDE SCIENCE, cilt.113, sa.1, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 113 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/pep2.24167
  • Dergi Adı: PEPTIDE SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: antimicrobial peptide, bacteria, fluorescent probe, fungi, optical imaging
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Peptides with pan-antimicrobial affinity were synthesized and decorated with environmentally sensitive fluorophores nitrobenzoxadiazole (green) and merocyanine (red). The labeling efficacies against a range of clinically relevant fungal and Gram-negative and positive bacterial species (Candida albicans, Escherichia coli, and Staphylococcus aureus) were explored. The fluorogenic probes containing exclusively l or d-amino acids showed rapid and efficient labeling of all microbial species, whereas probes with a mixture of l or d-amino acids failed to label fungi or bacteria, highlighting the importance of the alpha-helical peptide structure for interaction with the microbial cell membrane. Importantly, the nature of the dye allowed fluorescence detection/labeling without the need for a wash step, paving the way for direct application of the probes.