The aim of this study was to investigate Turkish college students' learning approach regarding biology and ecology classes along with the effects of some selected variables (gender, teacher, year of study, grade in biology and grade in ecology) on students' learning approach. The Turkish version of the Learning Approach Questionnaire was utilized for gathering data. The sample consisted of 173 female and 238 male (411) university students from two different departments at three different universities in Turkey. In this investigation, no statistically significant differences in terms of gender, teacher, and year of study, grade in biology and grade in ecology were found. Inferential statistics, mainly ANOVA, were used for testing the effects of selected variables on their learning approach.