Enhancement of Corchorus olitorius L. on osteogenic differentiation of MC3T3-E1 pre-osteoblast cells by increasing alkaline phosphatase and hydroxyproline

Ertugruloglu P., Baris E., Okkali G. S., SARIKAHYA N.

Natural Product Research, 2025 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2025
  • Doi Number: 10.1080/14786419.2025.2568205
  • Journal Name: Natural Product Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, CINAHL, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: <italic>Corchorus olitorius</italic> L., ALP, HYP, osteogenic differentiation
  • Dokuz Eylül University Affiliated: Yes

Abstract

Corchorus olitorius L. (jute mallow or molehiya) belongs to the Malvaceae family valued for its nutritional and medicinal properties. In this study, the potential to enhance osteogenesis in MC3T3-E1(Murine Calvaria-derived 3T3 Subclone E1) pre-osteoblastic cells was investigated to support bone formation and mineralisation. Leaf ethanolic extract was prepared and applied to MC3T3-E1 cells. Osteogenic effects were evaluated through three methods: MTT assays for cell viability, Alizarin Red S staining for calcium deposition, enzymatic analyses for alkaline phosphatase (ALP) and hydroxyproline (HYP). A non-cytotoxic concentration of C. olitorius extract (0.5 mg/mL) significantly increased ALP and HYP levels, promoting osteogenic differentiation in both undifferentiated and differentiated cells. HYP levels were notably elevated in differentiated cells. The findings suggested that C. olitorius extract may be a promising natural agent for enhancing bone health, warranting further in vivo and clinical studies to confirm its therapeutic potential.