Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

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Polat P. F., Sahan A., Aksoy G., Timurkan M. Ö., Dincer E.

ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH, vol.86, no.1, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 86 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.4102/ojvr.v86i1.1734
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Canine Parvovirus variants, molecular characterisation, Turkey, RFLP, restriction fragment length polymorphism, NEUTRALIZING ANTIBODY-RESPONSES, VP2 GENE, STRAINS, TYPE-2, VACCINATION, PUPPIES, EMERGENCE, EVOLUTION, EMPHASIS, VARIANT
  • Dokuz Eylül University Affiliated: Yes


Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey's dog population.