Assessment of oxidative DNA damage, apoptosis and histopathological alterations on zebrafish exposed with green silver nanoparticle
CHEMISTRY AND ECOLOGY, vol.38, no.7, pp.655-670, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 38 Issue: 7
- Publication Date: 2022
- Doi Number: 10.1080/02757540.2022.2108808
- Journal Name: CHEMISTRY AND ECOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Food Science & Technology Abstracts, Geobase, Pollution Abstracts, Veterinary Science Database
- Page Numbers: pp.655-670
- Keywords: Zebra fish, bax, degeneration, necrosis, 8-OHdG, nanoparticle
- Dokuz Eylül University Affiliated: Yes
Abstract
The effect of green synthesis approaches in NP synthesis and their possible toxicity in aquatic environments has been very limitedly studied. In this study, VA-Ag / AC NPs were synthesised using Viscum album plant as a reducing agent and Chenopodium album (CA) plant as an active carbon source. TEM (Transmission Electron Microscope) and XRD (X-ray crystallography) analysis of the synthesised VA-Ag / AC NPs were performed to detect their morphological and chemical properties. The inducing of oxidative stress and the effectiveness of VA-Ag/AC NPs in neurotoxic pathways and teratogenic changes in aquatic organisms have been investigated. In addition, a modelling was created to elucidate the toxicity mechanism. The results revealed that green synthesised VA-Ag/AC NPs nanoparticles are approximately 1600 times less toxic than nanoparticles synthesised by different methods. It had been determined that only high doses of VA-Ag/AC NPs nanoparticles cause neurological, histopathological and morphological changes. The findings in this study for VA-Ag/AC NPs, which are the evidence of the complexity of their mode of action at the cellular level, are a first in the aquatic ecosystem and require different findings regarding the stability and safety of such synthesis products.