Assessment of oxidative DNA damage, apoptosis and histopathological alterations on zebrafish exposed with green silver nanoparticle

Kokturk M., Yildirim S., ATAMANALP M., ÇALIMLI M. H., NAS M. S., BOLAT İ., ...More

CHEMISTRY AND ECOLOGY, vol.38, no.7, pp.655-670, 2022 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 7
  • Publication Date: 2022
  • Doi Number: 10.1080/02757540.2022.2108808
  • Journal Name: CHEMISTRY AND ECOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Food Science & Technology Abstracts, Geobase, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.655-670
  • Keywords: Zebra fish, bax, degeneration, necrosis, 8-OHdG, nanoparticle, BAX, ANTIBACTERIAL, STRESS, BRAIN, BCL-2
  • Dokuz Eylül University Affiliated: Yes

Abstract

The effect of green synthesis approaches in NP synthesis and their possible toxicity in aquatic environments has been very limitedly studied. In this study, VA-Ag / AC NPs were synthesised using Viscum album plant as a reducing agent and Chenopodium album (CA) plant as an active carbon source. TEM (Transmission Electron Microscope) and XRD (X-ray crystallography) analysis of the synthesised VA-Ag / AC NPs were performed to detect their morphological and chemical properties. The inducing of oxidative stress and the effectiveness of VA-Ag/AC NPs in neurotoxic pathways and teratogenic changes in aquatic organisms have been investigated. In addition, a modelling was created to elucidate the toxicity mechanism. The results revealed that green synthesised VA-Ag/AC NPs nanoparticles are approximately 1600 times less toxic than nanoparticles synthesised by different methods. It had been determined that only high doses of VA-Ag/AC NPs nanoparticles cause neurological, histopathological and morphological changes. The findings in this study for VA-Ag/AC NPs, which are the evidence of the complexity of their mode of action at the cellular level, are a first in the aquatic ecosystem and require different findings regarding the stability and safety of such synthesis products.