Biocompatibility of plasma-treated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofiber mats modified by silk fibroin for bone tissue regeneration

Unalan I., ÇOLPANKAN GÜNEŞ O., ZİYLAN A., Gorgun C., Urkmez A. S.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol.68, pp.842-850, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 68
  • Publication Date: 2016
  • Doi Number: 10.1016/j.msec.2016.07.054
  • Journal Name: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.842-850
  • Keywords: Nanofiber, Electrospinning, PHBV, Plasma surface modification, Silk fibroin, SURFACE MODIFICATION, CELL AFFINITY, SCAFFOLDS, FABRICATION, IMPROVEMENT, FILMS, PHBV
  • Dokuz Eylül University Affiliated: Yes

Abstract

The objective of this study was to produce biocompatible plasma-treated and silk-fibroin (SF) modified poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber mats. The mats were plasma-treated using O-2 or N-2 gas to increase their hydrophilicity followed by SF immobilization for the improvement of biocompatibility. Contact angle measurements and SEM showed increased hydrophilicity and no disturbed morphology, respectively. Cell proliferation assay revealed that SF modification together with N-2 plasma (PS/N-2) promoted higher osteoblastic (SaOs-2) cell viability. Although, O-2 plasma triggered more mineral formation on the mats, it showed poor cell viability. Consequently, the PS/N-2 nanofiber mats would be a potential candidate for bone tissue engineering applications. (C) 2016 Elsevier B.V. All rights reserved.