Biocompatibility of plasma-treated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofiber mats modified by silk fibroin for bone tissue regeneration

Unalan I., ÇOLPANKAN GÜNEŞ O., ZİYLAN A., Gorgun C., Urkmez A. S.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, cilt.68, ss.842-850, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.msec.2016.07.054
  • Dergi Adı: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.842-850
  • Anahtar Kelimeler: Nanofiber, Electrospinning, PHBV, Plasma surface modification, Silk fibroin, SURFACE MODIFICATION, CELL AFFINITY, SCAFFOLDS, FABRICATION, IMPROVEMENT, FILMS, PHBV
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

The objective of this study was to produce biocompatible plasma-treated and silk-fibroin (SF) modified poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber mats. The mats were plasma-treated using O-2 or N-2 gas to increase their hydrophilicity followed by SF immobilization for the improvement of biocompatibility. Contact angle measurements and SEM showed increased hydrophilicity and no disturbed morphology, respectively. Cell proliferation assay revealed that SF modification together with N-2 plasma (PS/N-2) promoted higher osteoblastic (SaOs-2) cell viability. Although, O-2 plasma triggered more mineral formation on the mats, it showed poor cell viability. Consequently, the PS/N-2 nanofiber mats would be a potential candidate for bone tissue engineering applications. (C) 2016 Elsevier B.V. All rights reserved.