The effects of granulocyte-colony stimulating factor (G-CSF) on lungs in a rat intra-abdominal sepsis model Ratlarda intra-abdominal sepsis modelinde uygulanan granulosit koloni- stimulan faktorun akcigerler uzerine etkisi


KAR A., Ozzeybek D., ÖZTEKİN S., KOCA U., TUNCEL E. P., ÖZER E., ...More

Turk Anesteziyoloji ve Reanimasyon, vol.28, no.3, pp.125-134, 2000 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 3
  • Publication Date: 2000
  • Journal Name: Turk Anesteziyoloji ve Reanimasyon
  • Journal Indexes: Scopus
  • Page Numbers: pp.125-134
  • Keywords: Granulocyte-colony stimulating factor, Intra-abdominal sepsis, Lung injury, Myeloperoxidase, Neutrophil, Tumor necrosis factor
  • Dokuz Eylül University Affiliated: Yes

Abstract

We investigated the effects of recombinant human granulocyte-colony stimulating factor (rhG-CSF) on serum levels of tumor necrosis factor (TNF) and on neutrophil infiltration in the lungs of rats undergoing cecal ligation and puncture as a method of sepsis induction. Thirty Wistar rats weighing between 200-220 g were entered into the study. Blood neutrophil count, serum TNF-α level, myeloperoxidase (MPO) activity in lung tissue, and lung histopathology were evaluated in six controls. The effects of surgical intervention on these parameters were investigated in a sham operation group (n=6); specimens were taken twelve hours post-op. The rhG-CSF group (n=6) was given 25 μg/kg of rhG-CSF subcutaneously, and specimens were taken eleven hours later. In the sepsis group, specimens of blood and tissue were obtained twelve hours after sepsis induction. In the sepsis rhG-CSF group, 25 μg/kg SC rhG-CSF was given after sepsis induction, then specimens were taken twelve hours post-op. Blood neutrophil count was increased and serum TNF-α decreased in the rhG-CSF groups compared to the control and other groups. MPO activity in lung tissue was found to be increased in all groups, and was greater in the sepsis and sepsis+rhG-CSF groups when compared with the sham group. On histopathological examination, the sham and sepsis+rhG-CSF groups had significantly more neutrophilic infiltration than the control group. No differences were found between the amounts of edema, hemorrhage or necrosis in the lungs of the sepsis and sepsis+rhG-CSF groups. In summary, rhG-CSF had no effect on neutrophil infiltration in the lungs of rats in this intra- abdominal sepsis model.