Equid herpesvirus 1 (EHV-1) is a major agent of large-scale outbreaks of abortion, and these abortions have been described as sporadic or epidemic cases in mares, generally during the last trimester of pregnancy. In this study, the partial characterization based on the glycoprotein B (gB) gene of Turkish (TR) EHV-1 field strains isolated from an abortion outbreak during the 2011 foaling season in Turkey was investigated using a novel designed primer set for EHV-1. The molecular analysis of TR EHV-1 strains showed that genetically identical TR EHV-1 strains were still circulating in Turkey during different years and, these strains were closely related to the European EHV-1 strains. Furthermore, EHV-1 isolated in the present study and to EHV-1 strains published previously in the GenBank database were shown some differences for the aa sequences. This molecular report would be valuable for monitoring of EHV-1 infection in Turkey and determining the gB gene sequence of newly identified EHV-1 field strains from future outbreaks on the pathogenesis and severity of disease.