Searching the Role of AKT Expression on Adiponectin Induced Cardiac Cells

Şimşek Papur Ö.

49. FEBS congress, İstanbul, Turkey, 5 - 09 July 2025, pp.60257, (Summary Text)

  • Publication Type: Conference Paper / Summary Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.60257
  • Dokuz Eylül University Affiliated: Yes

Abstract

Diabetic Cardiomyopathy (DCM) refers to abnormalities in the structure and function of the myocardium resulting from diabetes. In the healthy heart, glucose and long-chain fatty acids are used equally as energy sources. There is a dynamic balance in their uptake and transport. Adiponectin (APN) is an adipokine synthesized and secreted by adipose tissue. APN concentration is reduced in insulin resistance and type 2 diabetes. APN prevents insulin resistance by increasing fatty acid excretion and glucose by skeletal muscle. Insulin-activated phosphatidylinositol 3′-kinase-dependent serine/threonine kinase AKT functions as a regulator of glucose transport, glycolysis, glycogen synthesis, suppression of gluconeogenesis, protein synthesis, lipogenesis, cell survival, cell size determination, and cell cycle progression. Accumulation of AKT in the plasma membrane of insulin-stimulated adipocytes results in different substrate specificity and involvement in the regulation of GLUT4 transport. Activated AKT converts to p-AKT which translocates to the nucleus to activate its substrates. p-AKT can phosphorylate a wide range of proteins. The aim of this study is to search the role of AKT Expression on adiponectin induced cardiac cells in vitro. The cardiac characteristics of H9c2 cells were confirmed through immunofluorescence staining followed by fluorescence microscopy imaging of muscle-specific desmin intermediate filaments, cardiac-specific myosin  and perinuclear Atrial Natriuretic Factor (ANF). DCM model was created using the H9c2 cell line and time course experiment were made with different concentration level of adiponectin and examine the expression change of Akt by Western Blot method.Immunofluorescence study was showed H9c2 cell line’s cardiac behavior. Time-dependent treatment of APN in different concentrations to H9c2 cells resulted in changes in AKT expressions.This study aims to define new mechanisms that play a role in the pathogenesis of Diabetic cardiomyopathy.