Role of Copper Transporting ATPases in Contraction İnduced Cardiac Cell Lines

Şimşek Papur Ö.

50.FEBS Congress, Maastricht, Hollanda, 4 - 08 Temmuz 2026, ss.1-2, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Maastricht
  • Basıldığı Ülke: Hollanda
  • Sayfa Sayıları: ss.1-2
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Abstract

Rationale: Diabetic cardiomyopathy (DCM) is characterized by structural and functional abnormalities of the myocardium caused by diabetes mellitus. Oligomycin is a pharmacological agent commonly used to mimic muscle contraction by inhibiting mitochondrial ATP synthase, thereby simulating the energetic stress associated with cardiac contraction. Although copper plays essential roles in cardiac cell function, the involvement of copper transporters under diabetic conditions remains poorly understood. This study aimed to investigate the effects of copper and contraction-mimicking conditions on the expression of copper-transporting ATPase genes in cardiac cell lines under experimental settings modeling diabetic cardiomyopathy.

Methods: An experimental design incorporating different glucose concentrations was used to mimic diabetic conditions in cardiac cell lines. Oligomycin treatment was applied to simulate contraction-related stress. The expression levels of copper transporter genes were analyzed using RT-PCR and Western blotting.

Results: The results demonstrated that the expression levels of copper transporters were increased under diabetic conditions compared to the control group, both in the presence of oligomycin and copper treatments. Gene expression was also modulated according to extracellular copper concentrations.

Conclusions: Our findings indicate that copper-transporting ATPase expression is altered under contraction-mimicking diabetic conditions in cardiac cell lines. The expression profile is influenced by oligomycin treatment, glucose levels, and copper availability.