A Review on Color Normalization and Color Deconvolution Methods in Histopathology

Onder D., Zengin S., SARIOĞLU S.

APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, vol.22, no.10, pp.713-719, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 22 Issue: 10
  • Publication Date: 2014
  • Doi Number: 10.1097/pai.0000000000000003
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.713-719
  • Keywords: bright field microscopy, color deconvolution, color normalization, histopathology, microscopy, QUANTIFICATION
  • Dokuz Eylül University Affiliated: Yes


The histopathologists get the benefits of wide range of colored dyes to have much useful information about the lesions and the tissue compositions. Despite its advantages, the staining process comes up with quite complex variations in staining concentrations and correlations, tissue fixation types, and fixation time periods. Together with the improvements in computing power and with the development of novel image analysis methods, these imperfections have led to the emerging of several color normalization algorithms. This article is a review of the currently available digital color normalization methods for the bright field histopathology. We describe the proposed color normalization methodologies in detail together with the lesion and tissue types used in the corresponding experiments. We also present the quantitative validation approaches for each of the proposed methodology where available.