Sampling number effects on genetic variation analysis in maize landraces using seed and leaf tissues

KAHRIMAN F., EGESEL C. Ö., Songur U., ALACA YILDIRIM E.

Genetic Resources and Crop Evolution, cilt.72, sa.Suppl 1, ss.697-715, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: Suppl 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s10722-025-02566-x
  • Dergi Adı: Genetic Resources and Crop Evolution
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Geobase, Veterinary Science Database
  • Sayfa Sayıları: ss.697-715
  • Anahtar Kelimeler: Microsatellite, Polymerase chain reaction, Cluster analysis, <italic>Zea mays</italic>
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Maize landraces are crucial genetic resources for enhancing genetic diversity within breeding programs and for providing novel alleles that may be absent in registered cultivars. This study evaluates the effects of sample type (leaf vs. seed) and sample size (Single, 10, 20, and 30 individuals) on the outcomes of molecular diversity analysis in maize. DNA was extracted from 11 different maize landraces and two standard genotypes (B73 and Mo17) using optimized protocols. Simple sequence repeat (SSR) marker analysis revealed significant variation in genetic diversity indices between sample types. The analysis of molecular of variance (AMOVA) indicated that 10.98% of the variation in seed samples was explained by the sampling method, while all variation in leaf samples was attributed to differences among genotypes. Dendrogram and graphical analyses demonstrated that seed samples from groups of 10 and 20 exhibited more genetic similarity, while leaf samples showed higher complexity between 10 and 30-sample groups. These findings support the utility of SSR markers in evaluating genetic diversity and emphasize that both tissue type and sample size should be carefully considered in future assesments of genetic variability in maize landraces.