Comparison of Different Staining Methods for Detecting Blastocystis hominis Cysts


Yalcin G., Delibas S. B.

TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, vol.29, no.6, pp.1370-1375, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 6
  • Publication Date: 2009
  • Journal Name: TURKIYE KLINIKLERI TIP BILIMLERI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1370-1375
  • Keywords: Blastocystis hominis, protozoa, INFECTION
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: Blastocystis hominis (B. hominis) is the most common intestinal parasite in humans and is also present in a wide range of animal hosts. B hominis is a polymorphic organism that can be seen in a wide geographic distribution, and it is mostly detected by three morphologic forms. Cystic stage that supposed to be the infective form of the parasite was confirmed and described recently. The aim of the present study is to identify the B. hominis cyst using the different staining methods. Material and Methods: Thirty one stool samples with B. hominis which was detected by saline-iodine or trichrome staining method were included in the study. Alcian blue and toludine blue stained smears, prepared after the Ficoll-Paque concentration method, were examined for the B. hominis cysts. Results: 70.6% of the patients had complaints as diarrhea, abdominal pain and nausea. The cystic stages were generally measured as 3-10 mu m. Cysts were spherical or ovoid and they were stained red-pink with trichrome staining, royal blue with toluidine blue, and stained pink with alcian blue. We determined the presence of B. hominis cysts 96.6% with trichrome, 84.6% with toluidine blue and 76.0% with alcian blue staining. In one of the stool samples no cystic stage was observed with any of the staining methods. Conclusion: As a result, in this study we showed that B. hominis cystic stages could have been detected by diverse staining methods after the concentration technique. We also showed that the cystic stages mostly accompany other forms. This is the first study in our country that isolated and recognized the cystic stage of the B. hominis which is the resistant form of the parasite that maintains the survival of the parasite in the environment.