M3: an integrative framework for structure determination of molecular machines

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KARACA EREK E., Rodrigues J. P. G. L. M., Graziadei A., Bonvin A. M. J. J., Carlomagno T.

NATURE METHODS, cilt.14, sa.9, ss.897-905, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 9
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1038/nmeth.4392
  • Dergi Adı: NATURE METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.897-905
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

We present a broadly applicable, user-friendly protocol that incorporates sparse and hybrid experimental data to calculate quasi-atomic-resolution structures of molecular machines. The protocol uses the HADDOCK framework, accounts for extensive structural rearrangements both at the domain and atomic levels and accepts input from all structural and biochemical experiments whose data can be translated into interatomic distances and/or molecular shapes.