Phenolic Profiles, Antimicrobial and Cytotoxic Properties of Both Micropropagated and Naturally Growing Plantlets of <i>Calamintha sylvatica</i> subsp. <i>sylvatica</i> Bromf.

Cuce M., Bekircan T., Laghari A. H., Sokmen M., Sokmen A., ÖNAY UÇAR E., ...Daha Fazla

NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, cilt.47, sa.4, ss.1145-1152, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 4
  • Basım Tarihi: 2019
  • Doi Numarası: 10.15835/nbha47411474
  • Dergi Adı: NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1145-1152
  • Anahtar Kelimeler: antimicrobial activity, Calamintha sylvatica, cytotoxic activity, micropropagation, rosmarinic acid
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

A rapid micropropagation protocol was designed to produce Calamintha sylvatica plantlets by using nodal segments as explants for the shoot formation. 6-BA favored the highest shoot formation and biomass yield, whilst kinetin was found superior for the highest shoot length (38.97 +/- 2.85 mm) and node numbers (2.89 +/- 0.63). Rosmarinic acid was detected as major phenolic acid, ranging from 7.59 mg/100 g to 81.44 mg/100 g. Hexane extracts from natural and in vitro propagated plantlets showed activity only against Staphylococcus aureus ATCC 25923 with MIC values at 6.25 and 3.33 m/mL, respectively while in the latter case, extracts from natural plantlets exerted higher cytotoxic activity than those of micropropagated ones (IC50 values were 83 mu g/mL and 98 mu g/mL on HeLa cells, respectively). C. sylvatica showed high micropropagation performance and produced remarkable amount of rosmarinic acid in vitro as well as antimicrobial and cytotoxic effect.