Tc-99m-HMPAO uptake by bronchoalveolar cells


Durak H., Kilinc O., Ertay T., Ucan E. S., Kargi A., ÇAPA KAYA G., ...More

ANNALS OF NUCLEAR MEDICINE, vol.17, no.2, pp.107-113, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 2
  • Publication Date: 2003
  • Doi Number: 10.1007/bf02988447
  • Journal Name: ANNALS OF NUCLEAR MEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.107-113
  • Keywords: Tc-99m-HMPAO, bronchoalveolar lavage, radioaerosols, alveolar macrophages, smoking, CIGARETTE SMOKERS, LUNG UPTAKE, GLUTATHIONE, HMPAO, CLEARANCE, TECHNETIUM-99M-MESO-HMPAO, SCINTIGRAPHY, INJURY, AGENT
  • Dokuz Eylül University Affiliated: Yes

Abstract

Lung uptake of intravenously injected Tc-99m-HMPAO is observed in smokers and in lung toxicity due to various agents. We investigated the Tc-99m-HMPAO uptake of bronchoalveolar lavage (BAL) cells in the lungs after incubation in in vitro conditions (6 patients), intravenous injection (IV) (7 patients) and inhalation (INH) (6 patients) of Tc-99m-HMPAO in order to show whether BAL cells are also responsible for Tc-99m-HMPAO uptake in the lungs. Cell/supernatant (C/S) count ratio was 7.0 +/- 3.5, 29.3 +/- 40.8 and 8.4 +/- 4.5 for in vitro, IV and INH groups, respectively. C/S-in vitro showed a positive correlation with % alveolar macrophages (r = 0.943, p = 0.0048) and a negative correlation with % neutrophils (r = -0.945, p = 0.0045). Cells/whole BAL fluid ratio cot-related with the amount of daily cigarette consumption in INH group (r = 0.95, p = 0.0037). Tc-99m-HMPAO showed adherence to mucus after inhalation. Tc-99m-HMPAO diffuses into alveolar spaces after injection and is present in BAL fluid and BAL cells both after injection and inhalation. Glutathione concentration and oxido-reductive state of the epithelial lining fluid and BAL cells may influence the lung uptake of Tc-99m-HMPAO.